Featured Documentation

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Thermo Fisher KingFisher Apex system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on Hamilton NIMBUS Presto system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Apex system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Duo Prime system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Flex system

Procedure & checklist – Extracting HMW DNA from standard Dounce homogenizer tissue using Nanobind kits

Description

This procedure can be used to extract HMW DNA from animal tissues.

Procedure & checklist – Extracting HMW DNA from plant nuclei using Nanobind kits

Description

This procedure can be used to extract HMW DNA from plant nuclei.

Procedure & checklist – Isolating nuclei from plant tissue using TissueRuptor disruption

Description

This procedure can be used to isolate nuclei from plant tissues for HMW DNA extraction using the Nanobind plant nuclei kit